Quantification of hydroxyl radical generation with spin trapping ESR technique in batch system
نویسندگان
چکیده
منابع مشابه
Signal enhancement in ESR spin-trapping for hydroxyl radicals.
In an ESR spin-trapping measurement of hydroxyl radicals formed in a Fenton reaction, the trapping efficiency with DMPO increased by about 300 times in a sodium trifluoroacetate solution, whereas it was little changed in a phosphate buffer.
متن کاملESR spin trapping of hydroxyl radicals in aqueous solution irradiated with high-LET carbon-ion beams.
The aim of this study was to quantify the hydroxyl radicals (*OH) produced when aqueous solutions are decomposed by high-linear energy transfer (LET) 290 MeV/nucleon carbon-ion beams using an electron spin resonance (ESR) spectrometer. Aerated cell culture medium containing 200 mM 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was irradiated with doses of 0 to 20 Gy with an LET of 20 to 90 keV/ micro ...
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Electron spin resonance spectroscopy has been used to study free radical generation in rats with acute sodium formate poisoning. The in vivo spin-trapping technique was used with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN), which reacts with free radical metabolites to form radical adducts, which were detected in the bile and urine samples from Fischer rats. The use of [(13)C]-sodium form...
متن کاملAn Advanced Electron Spin Resonance (ESR) Spin-Trapping and LC/(ESR)/MS Technique for the Study of Lipid Peroxidation
There are two types of nutritionally important polyunsaturated fatty acids (PUFAs), namely ω-6s and ω-3s. PUFAs and their metabolites generated from lipid peroxidation via cyclooxygenase (COX) and lipoxygenase (LOX) are believed to be involved in a variety of physiological and pathological processes in the human body. Both COX- and LOX-catalyzed PUFA peroxidation are complex events th...
متن کاملSpin-trapping and human neutrophils. Limits of detection of hydroxyl radical.
Using the spin trap, 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) and an excess of dimethyl sulfoxide, we previously reported that in the absence of an exogenous iron catalyst, human neutrophils will not generate hydroxyl radical, manifested as the catalse-inhibitable methyl radical spin-trapped adduct, 2,2,5-trimethyl-1-pyrrolidinyloxy (DMPO-CH3) (Britigan, B. E., Rosen, G. M., Chai, Y., and Cohen,...
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ژورنال
عنوان ژورنال: Journal of Water and Environment Technology
سال: 2003
ISSN: 1348-2165
DOI: 10.2965/jwet.2003.43